The emergence of the CRISPR/Cas9 system marks a pivotal moment in genome engineering. This innovative technology facilitates precision genome editing and transcriptional regulation through the use of a chimeric single guide RNA (sgRNA), guiding the Cas9 protein to specific DNA sequences. By swapping out a mere 20 base pair protospacer within the sgRNA, researchers can target diverse DNA sequences, making this system versatile for a variety of applications. Its ability to simultaneously target multiple genomic loci via co-expression of a Cas9 protein with multiple sgRNAs underscores its suitability for multiplex gene editing and synergistic gene activation.

At Creative Biogene, we take pride in offering the QVirus™ Platform, meticulously designed for the rapid generation of lentiviral Cas9 constructs tailored for multiplex gene manipulation. This platform not only streamlines the process of multiplex gene editing but also enables transient or sustained transcriptional activation in human cells. The lentiviral CRISPR/Cas9 system proves invaluable in both fundamental research and therapeutic applications by facilitating highly efficient and customizable genomic manipulations in cell types that are notoriously challenging to transfect, such as primary cells and progenitor cells. Furthermore, the versatility of our vectors supports modeling complex diseases resulting from multiple mutations.

Why Choose QVirus™ Platform for Your CRISPR Needs?

Implementing our lentiviral-based CRISPR constructs can guarantee permanent knockout efficiency within your cell lines. With stable expression of sgRNA and Cas9, this service is suitable for both dividing and non-dividing cells, as well as whole model organisms. We provide both Single-Vector and Two-Vector CRISPR-Cas9 system services.

Single-Vector CRISPR-Cas9 System

• This system negates the need for co-transduction, as both sgRNA and Cas9 nuclease are expressed within a single vector.
• Our lentiviral vectors include antibiotic selection markers to ensure stability.
• These vectors integrate seamlessly into the host cell genome, ensuring they are passed down through subsequent cell divisions.
• Cells can be treated, grown for extended passages, or frozen and thawed, with the lentiviral construct remaining within the host cells throughout.

Two-Vector CRISPR-Cas9 System

• Initial transduction with Cas9 lentivirus minimizes noise within knockout screens.
• Options for Tet-inducible and constitutive U6 expression are available.
• High-titer sgRNA lentivirus is produced to enhance transduction efficiency.
• Several antibiotic resistance genes are included in the vectors to promote stability and facilitate selection.

Advantages of QVirus™ Platform

• High-efficiency delivery of Cas9, accompanied by markers for tracking purposes.
• Elimination of tedious cloning or vector construction efforts.
• Outstanding nuclear penetration capability for the Cas9 enzyme.
• Capacity to conduct multiple gene editing tasks simultaneously.

The QVirus™ Platform excels in producing substantial yields of lentiviruses carrying essential components for CRISPR/Cas9-mediated genome editing, including sgRNA and Cas9 nuclease. This is particularly advantageous for reaching mammalian cells that are difficult to transfect. For any specific requirements or inquiries, our team is readily available to assist.