Business Management

PAMPA Introduction

  • Diffusion and absorption of drugs

Drug permeability is one of the most important factors to be considered for predicting oral drug bioavailability. Permeation mechanisms through biological barriers include active transport, passive diffusion, paracellular, and efflux. The body absorbs nearly 80~95% of the recorded commercial drugs through passive diffusion.

For permeability screening, the Caco-2 cell monolayer permeation method has been widely and successfully used in drug discovery and early development. With the need for reduced cost, increased predictability, and higher throughput in drug discovery, other methods are needed to assist Caco-2 assay.

  • Why PAMPA?

PAMPA uses two aqueous buffer solution holes separated by an artificial membrane. The artificial membrane is composed of a lipid-oil-lipid sandwich structure in an organic diluent supported by a porous filter plate matrix. And then, the test compound diluted in the buffer is placed in the donor well. The compound enters the artificial membrane from the donor pore, entering the acceptor pore by passive diffusion. The compound's effective permeability (Pe) is utilized to determine the rate of permeation. Compared with cell-monolayer methods, the time required for the experiment is greatly reduced. Only passive diffusion is tested, and there is no metabolism, no transporter proteins, so there is no need to worry about saturation.


Courses tagged with "Business Management"

Understanding Fee Setting

Pricing is a key component of business. The pricing of products and services has a significant influence on how profitable a practice will be. Pricing is integral to your overall business strategy, and the positioning of the practice in the marketplace. Learn about fee setting approaches, the processes and methods to use, types of costs, mark-up vs margin and more.

Course Type:
Interactive lessons that should take up to 1 hour to complete

Features:
You have access to the course for 365 days
Includes downloadable resources
Complete interactive lessons to attain your certificate of achievement

CPD Points:
1 AVNAT Point